10ksgt6ss

Effector Repertoire of Salmonella T6SS

📰 Citation

Nicastro, G.G., Sibinelli-Sousa, S., Hespanhol, J.T., et al. (2025) Genome-directed study reveals the diversity of Salmonella T6SS effectors and identifies a novel family of lipid-targeting antibacterial toxins. bioRxiv.https://doi.org/10.1101/2024.09.27.615498

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🔎 How to Use This Resource

This page provides curated information on Salmonella T6SS effector candidates identified in our genome-wide analysis. Each row in the table represents a specific effector group (e.g., STox_2), with links to the relevant data and structural models. Each table on this page represents a broader thematic category of effector activity, grouping together toxins with similar molecular targets or biochemical mechanisms. You can navigate directly to each category below:

• Nuclease (DNase, RNase)
• Deaminase
• Peptidase
• ADP-ribosyltransferase
• Glycosidase
• Phospholipase and Acyltransferase
• Pore-forming
• Adenylyl cyclase
• Phosphodiester / Glycosidase / NagPA-like
• Not determined

Each section contains a curated table of candidate effectors, their alignments, structural models, and predicted functions. Columns include:

• Model: HMM model constructed in this study.
• BLAST: Link to run NCBI BLASTP using the reference sequence (first sequence in the alignment) as query against the NCBI nr database.
• Alignments: Multiple sequence alignments used to build the HMMs, available in HTML (with and without gaps) and in FASTA format for download. All alignments include NCBI protein accession numbers.
• HHpred: HHpred remote homology search results obtained using the full alignment as input.
• Group: Functional or structural classification (e.g., BECR fold).
• AlphaFold: Access to AlphaFold2-predicted 3D structures, with options to visualize them using Mol* or download in .molx format.

Use the links in each row to explore and download sequences, alignments, and predicted structures.

Nuclease: DNase, RNase

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
STox_2	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
STox_6	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
STox_11	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
STox_37	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
STox_57	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
STox_58	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
STox_59	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
STox_60	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
Ntox19.st	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
Ntox18.st1	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
Ntox18.st2	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
Ntox49.st	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
Tox-Ribonuclease.st	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
Ntox47.st2	pdf file	blast	without gap, with gap, download	HHpred	BECR	view download
STox_1	pdf file	blast	without gap, with gap, download	HHpred	BECR-like	view download
STox_3	pdf file	blast	without gap, with gap, download	HHpred	BECR-like	view download
STox_4	pdf file	blast	without gap, with gap, download	HHpred	BECR-like	view download
STox_55	pdf file	blast	without gap, with gap, download	HHpred	BECR-like	view download
Ntox7.st	pdf file	blast	without gap, with gap, download	HHpred	BECR-like	view download
Ntox47.st1	pdf file	blast	without gap, with gap, download	HHpred	BECR-like	view download
STox_36	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
STox_53	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
STox_61	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
Tke2.st	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
Tox-AHH.st1	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
Tox-AHH.st2	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
Tox-AHH.st3	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
Tox-AHH.st4	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
Tox-WHH.st1	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
Tox-WHH.st2	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
Tox-HNHGH-E.st	pdf file	blast	without gap, with gap, download	HHpred	HNH	view download
STox_54	pdf file	blast	without gap, with gap, download	HHpred	REase (PDDExK)	view download
Tox-REase-7.st	pdf file	blast	without gap, with gap, download	HHpred	REase (PDDExK)	view download
Tox-REase-3/4.st	pdf file	blast	without gap, with gap, download	HHpred	REase (PDDExK)	view download
TseV	pdf file	blast	without gap, with gap, download	HHpred	REase (PDDExK)	view download
CdiA_C.st	pdf file	blast	without gap, with gap, download	HHpred	REase (PDDExK)	view download
ParBL.st1	pdf file	blast	without gap, with gap, download	HHpred	ParB/Sulfiredoxin	view download
ParBL.st3	pdf file	blast	without gap, with gap, download	HHpred	ParB/Sulfiredoxin	view download
ParBL.st2	pdf file	blast	without gap, with gap, download	HHpred	ParB/Sulfiredoxin	view download
STox_7	pdf file	blast	without gap, with gap, download	HHpred	GIY-YIG	view download
Tox-URI1.st	pdf file	blast	without gap, with gap, download	HHpred	GIY-YIG	view download
Tox-URI2.st	pdf file	blast	without gap, with gap, download	HHpred	GIY-YIG	view download
STox_14	pdf file	blast	without gap, with gap, download	HHpred	Ribonuclease A	view download
Ntox34.st	pdf file	blast	without gap, with gap, download	HHpred	Not determined (All-α)	view download
Ntox44.st1	pdf file	blast	without gap, with gap, download	HHpred	Not determined (All-α)	view download
Ntox44.st2	pdf file	blast	without gap, with gap, download	HHpred	Not determined (All-α)	view download

Deaminase

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
STox_20	pdf file	blast	without gap, with gap, download	HHpred	Deaminase*	view download
ToxJAB2.st1	pdf file	blast	without gap, with gap, download	HHpred	Deaminase*	view download
ToxJAB2.st2	pdf file	blast	without gap, with gap, download	HHpred	Deaminase*	view download

Peptidase

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
STox_19	pdf file	blast	without gap, with gap, download	HHpred	Metallopeptidase	view download
STox_41	pdf file	blast	without gap, with gap, download	HHpred	Metallopeptidase	view download
STox_51	pdf file	blast	without gap, with gap, download	HHpred	Metallopeptidase	view download
Tox-HopH1.st	pdf file	blast	without gap, with gap, download	HHpred	Metallopeptidase	view download
Tox-Peptidase M4.st	pdf file	blast	without gap, with gap, download	HHpred	Metallopeptidase	view download
Tox-Peptidase M23.st	pdf file	blast	without gap, with gap, download	HHpred	Metallopeptidase	view download
Tox-Caspase.st	pdf file	blast	without gap, with gap, download	HHpred	Caspase-like	view download
Tlde1.st2	pdf file	blast	without gap, with gap, download	HHpred	LD-Peptidase	view download
Tlde1.st1	pdf file	blast	without gap, with gap, download	HHpred	LD-Peptidase	view download
STox_44	pdf file	blast	without gap, with gap, download	HHpred	Papain-like peptidase	view download
EvpQ.st	pdf file	blast	without gap, with gap, download	HHpred	Papain-like peptidase	view download
Tae2.st	pdf file	blast	without gap, with gap, download	HHpred	Papain-like peptidase	view download
Tae4.st	pdf file	blast	without gap, with gap, download	HHpred	Papain-like peptidase	view download

ADP-ribosyltransferase

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
STox_12	pdf file	blast	without gap, with gap, download	HHpred	ADP-ribosyltransferase	view download
STox_23	pdf file	blast	without gap, with gap, download	HHpred	ADP-ribosyltransferase	view download
STox_62	pdf file	blast	without gap, with gap, download	HHpred	ADP-ribosyltransferase	view download
TreTu.st	pdf file	blast	without gap, with gap, download	HHpred	ADP-ribosyltransferase	view download
RhsP2.st	pdf file	blast	without gap, with gap, download	HHpred	ADP-ribosyltransferase	view download
Tox-ART-HYD.st	pdf file	blast	without gap, with gap, download	HHpred	ADP-ribosyltransferase	view download
Tox-ART-HYD3.st	pdf file	blast	without gap, with gap, download	HHpred	ADP-ribosyltransferase	view download

Glycosidase

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
STox_42	pdf file	blast	without gap, with gap, download	HHpred	Lysozyme-like	view download
STox_46	pdf file	blast	without gap, with gap, download	HHpred	Lysozyme-like	view download
STox_56	pdf file	blast	without gap, with gap, download	HHpred	Lysozyme-like	view download
Tge2.st	pdf file	blast	without gap, with gap, download	HHpred	Lysozyme-like	view download
Pesticin.st	pdf file	blast	without gap, with gap, download	HHpred	Lysozyme-like	view download
STox_50	pdf file	blast	without gap, with gap, download	HHpred	Muramidase	view download

Phospholipase and Acyltransferase

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
Tox-Act1(STox_15)	pdf file	blast	without gap, with gap, download	HHpred	Papain-like peptidase	view download
TseH.st	pdf file	blast	without gap, with gap, download	HHpred	Papain-like peptidase	view download
Tle1.st	pdf file	blast	without gap, with gap, download	HHpred	α/β hydrolase	view download
Tle3.st	pdf file	blast	without gap, with gap, download	HHpred	α/β hydrolase	view download

Pore-forming

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
STox_17	pdf file	blast	without gap, with gap, download	HHpred	Colicin-like	view download
STox_18	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
VasX.st	pdf file	blast	without gap, with gap, download	HHpred	Colicin-like	view download
Ssp6.st	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download

Adenylyl cyclase

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
ExoY.st	pdf file	blast	without gap, with gap, download	HHpred	Polymerase β superfamily nucleotidyltransferase	view download

Phosphodiester glycosidase (NAGPA)

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
STox_35	pdf file	blast	without gap, with gap, download	HHpred	Phosphodiester glycosidase	view download

Not determined

Model	Genome context	blast	Alignment	HHpred	Group	AlphaFold
STox_24	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
STox_25	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
STox_30	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
STox_29	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
STox_31	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
STox_32	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
STox_33	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
STox_39	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
STox_45	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download
STox_49	pdf file	blast	without gap, with gap, download	HHpred	Not determined	view download